BMG POLARSTAR OMEGA PDF

Membrane fluidity is measured in both liposomes and mitochondrial membranes via fluorescence polarization UV FP optics are used on the POLARstar Omega to measure DPH 1,6-diphenyl-hexa-1,3,5-triene fluorescence The effects of dibucaine and propranolol on membrane fluidity are measured Introduction Membrane fluidity and other properties of lipids are commonly assessed using various fluorogenic membrane probes and fluorescence polarization FP measurements. The basic principle is that alterations in lipid packing e. FP is defined as the following ratio: are fluorescent intensities measured in the parallel and perpendicular channels, respectively. Membrane fluidization increases mobility of the dye and decreases the intensity of the emitted parallel component.

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Antioxidants are able to neutralize Reactive Oxygen Species Antioxidant capacity of fruit juices determined with the ORAC assay in 96 and well format MARS data analysis software with predefined templates for quick ORAC evaluation Introduction In all oxygen consuming cells, metabolism and oxidative stress generate several intermediates and byproducts that are collectively known as reactive oxygen species ROS.

ROS are dangerous to cellular structures and functional molecules i. DNA, proteins, lipids as they act as strong oxidizing agents or free radicals. Biological antioxidants are able to dispose of ROS; however, they are not completely effective in eliminating all of the free radicals, oxygen ions and peroxides that can do damage to the body. Furthermore, ROS can be generated from exposure to other external sources such as cigarette smoke, pollutants, chemicals and environmental toxins.

Different fruit juices were measured for their ORAC value. Data points are summarized over the time by the MARS data analysis software. Please refer to Application Note to see the assay principle graphically. All solutions should be prepared fresh daily. In every working well of a well plate the following was pipetted in triplicate.

Alternatively, incubation can be performed in the microplate reader. After incubation, fluorescence measurements ex. Alternatively, AAPH can also be added manually with a multi-channel-pipette. This has to be done as quickly as possible since the ROS-generator displays immediate activity after addition. The test was resumed and fluorescence intensity measurements were taken up to minutes. An instrument setting overview is given in application note Results and Discussion Fruit juices have ingredients that can quench the fluorescence signal.

This usually depends on the dilution used Fig. The degree of quenching varies between the different fruit juices and can cause problems in ORAC evaluation when the data is not normalized. The area under the signal curve is depending on the concentration of the antioxidant standard substance Fig. In case of quenching ingredients, that are still active even in high dilutions, all signal curves should be normalized.

Applying Curve Scaling will allow for optimal area under the curve calculation. In Table 1 a summary of all tested fruit juices is given. From the tested fruit juices the sallow thorn and blueberry ones show the highest ORAC values. Please notice that the values may differ when compared to similar measurements as juice ingredients are different from manufacturer to manufacturer.

The results obtained in both plate formats, well and well, are similar. Predefined templates in combination with curve scaling features give ORAC results quickly and easily. References Cao, G.

Free Radical Biol. Glazer, A. Methods Enzymol.

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ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader

This allows for a much higher signal-to-noise ratio than in competing readers and for lower limits of detection. BMG Labtech POLARstar Omega Microplate Readers The following main categories are amongst a wide range of possible applications: Filters are also the technically preferred and most Using this correction, data normalization to 1 cm path is given with only one mouse click. This is ideal for multi-excitation and multi-emission applications. Conforms to SBS standards for microplates. Open the catalog to page 6. This prevents condensation build-up on the lid or sealer.

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BMG POLARSTAR OMEGA PDF

Antioxidants are able to neutralize Reactive Oxygen Species Antioxidant capacity of fruit juices determined with the ORAC assay in 96 and well format MARS data analysis software with predefined templates for quick ORAC evaluation Introduction In all oxygen consuming cells, metabolism and oxidative stress generate several intermediates and byproducts that are collectively known as reactive oxygen species ROS. ROS are dangerous to cellular structures and functional molecules i. DNA, proteins, lipids as they act as strong oxidizing agents or free radicals. Biological antioxidants are able to dispose of ROS; however, they are not completely effective in eliminating all of the free radicals, oxygen ions and peroxides that can do damage to the body. Furthermore, ROS can be generated from exposure to other external sources such as cigarette smoke, pollutants, chemicals and environmental toxins. Different fruit juices were measured for their ORAC value.

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